1. Prepare your workspace: Make sure the area is clean and free from any potential contaminants. You can wipe down surfaces with a disinfectant to create a sterile environment.

2. Shake the syringe: Gently shake the liquid culture syringe to evenly distribute the contents inside. This ensures that the mycelium or fungal culture is well mixed.

3. Remove the cap: Carefully remove the cap from the syringe, taking care not to touch the exposed needle or any surfaces. Maintaining cleanliness is crucial to avoid contamination.

4. Inoculate the substrate: Identify the area or material where you want to introduce the liquid culture. This can be a sterilized growth medium or substrate suitable for the specific fungus you are cultivating.

5. Insert the needle: Insert the needle of the syringe into the chosen substrate. The depth of insertion will depend on the type of cultivation method you are following. Be gentle to avoid damaging the substrate.

6. Release the liquid culture: Depress the plunger slowly and steadily to release a small amount of liquid culture into the substrate. This introduces the fungal mycelium, which will grow and colonize the substrate.

7. Repeat the process: If needed, move to the next area or substrate and repeat steps 4 to 6 until you have inoculated all desired locations. Take care to maintain sterility throughout the process.

8. Store or incubate: Once you have finished inoculating, follow the specific instructions for the fungus you are cultivating. This may involve storing the inoculated substrate in a controlled environment with appropriate temperature and humidity conditions.

Remember, maintaining a clean and sterile environment is crucial when using liquid culture syringes to avoid contamination and promote successful fungal growth.